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 | Acceso al texto completo restringido a Biblioteca INIA La Estanzuela. Por información adicional contacte bib_le@inia.org.uy. |
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Registro completo
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Biblioteca (s) : |
INIA La Estanzuela. |
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Fecha : |
06/09/2021 |
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Actualizado : |
25/04/2022 |
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Tipo de producción científica : |
Artículos en Revistas Indexadas Internacionales |
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Autor : |
CLAVIJO, F.; CURLAND, R.D.; CROCE, V.; LAPAZ, M.I.; DILL-MACKY, R.; PEREYRA, S.; SIRI, M.I. |
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Afiliación : |
FELIPE CLAVIJO,, Universidad de la República Facultad de Química, 201894, Área Microbiología, Departamento de Biociencias, Montevideo, Montevideo, Uruguay.; REBECCA D. CURLAND,, University of Minnesota System, 311816, Plant Pathology, Minneapolis, Minnesota, United States.; VALENTINA CROCE, Universidad de la República Facultad de Química, 201894, Área Microbiología, Departamento de Biociencias, Montevideo, Montevideo, Uruguay.; MARÍA INÉS LAPAZ, Universidad de la República Facultad de Química, 201894, Área Microbiología, Departamento de Biociencias, Montevideo, Montevideo, Uruguay.; RUTH DILL-MACKY, University of Minnesota System, 311816, Plant Pathology, Minneapolis, Minnesota, United States.; SILVIA ANTONIA PEREYRA CORREA, INIA (Instituto Nacional de Investigación Agropecuaria), Uruguay; MARÍA INÉS INÉS SIRI, Universidad de la República Facultad de Química, 201894, Área Microbiología, Departamento de Biociencias, Montevideo, Montevideo, Uruguay. |
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Título : |
Genetic and phenotypic characterization of Xanthomonas species pathogenic of wheat in Uruguay. |
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Fecha de publicación : |
2022 |
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Fuente / Imprenta : |
Phytopathology, March 2022, vol. 112 (3): 511-520. Doi: https://doi.org/10.1094/PHYTO-06-21-0231-R |
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ISSN : |
e-ISSN:1943-7684 |
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DOI : |
10.1094/PHYTO-06-21-0231-R |
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Idioma : |
Inglés |
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Notas : |
Article history: Accepted for publication/ Posted online on 12 Aug 2021, First Look. |
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Contenido : |
Abstract: Bacterial diseases affecting wheat production in Uruguay are an issue of growing concern yet remain largely uninvestigated in the region. Surveys of 61 wheat fields carried out from 2017 to 2019 yielded a regional collection of 63 strains identified by 16S rRNA gene analysis as Xanthomonas spp. A real-time PCR protocol using species-specific primers previously reported allowed the identification of 44 strains as X. translucens, the causal agent of bacterial leaf streak (BLS) in wheat and other cereal crops. Multilocus sequence analysis (MLSA) of four housekeeping genes (dnaK, fyuA, gyrB, and rpoD) revealed that these strains were most closely related to X. translucens pv. undulosa, the pathovar that is most commonly associated with BLS of wheat. Multilocus sequence typing (MLST) was applied to examine the genetic diversity among X. translucens strains. Strains were assigned to four different sequence types, three of which have been previously reported globally. Additionally, 17 Xanthomonas strains not belonging to X. translucens were obtained from diseased wheat leaves. Phylogenetic analysis showed that these strains are closely related to Xanthomonas prunicola, and clustered together with previously uncharacterized Xanthomonas strains isolated from wheat in Minnesota, US. In planta pathogenicity assays carried out on a BLS susceptible wheat cultivar showed that X. translucens pv. undulosa strains caused brown necrosis symptoms typical of BLS, while non-translucens Xanthomonas sp. strains elicited an atypical symptom of dry necrosis. These findings suggest that local wheat fields are affected by X. translucens pv. undulosa, and by a new wheat pathogen within the Xanthomonas genus. MenosAbstract: Bacterial diseases affecting wheat production in Uruguay are an issue of growing concern yet remain largely uninvestigated in the region. Surveys of 61 wheat fields carried out from 2017 to 2019 yielded a regional collection of 63 strains identified by 16S rRNA gene analysis as Xanthomonas spp. A real-time PCR protocol using species-specific primers previously reported allowed the identification of 44 strains as X. translucens, the causal agent of bacterial leaf streak (BLS) in wheat and other cereal crops. Multilocus sequence analysis (MLSA) of four housekeeping genes (dnaK, fyuA, gyrB, and rpoD) revealed that these strains were most closely related to X. translucens pv. undulosa, the pathovar that is most commonly associated with BLS of wheat. Multilocus sequence typing (MLST) was applied to examine the genetic diversity among X. translucens strains. Strains were assigned to four different sequence types, three of which have been previously reported globally. Additionally, 17 Xanthomonas strains not belonging to X. translucens were obtained from diseased wheat leaves. Phylogenetic analysis showed that these strains are closely related to Xanthomonas prunicola, and clustered together with previously uncharacterized Xanthomonas strains isolated from wheat in Minnesota, US. In planta pathogenicity assays carried out on a BLS susceptible wheat cultivar showed that X. translucens pv. undulosa strains caused brown necrosis symptoms typical of BLS, while non-translucens... Presentar Todo |
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Palabras claves : |
Bacterial leaf streak; Bacterial Pathogens; Multilocus sequence analysis and typing; POPULATION BIOLOGY; WHEAT; Xanthomonas. |
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Thesagro : |
FITOPATOLOGIA; TRIGO; TRITICUM AESTIVUM; URUGUAY. |
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Asunto categoría : |
H20 Enfermedades de las plantas |
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Marc : |
LEADER 02834naa a2200349 a 4500
001 1062388
005 2022-04-25
008 2022 bl uuuu u00u1 u #d
022 $ae-ISSN:1943-7684
024 7 $a10.1094/PHYTO-06-21-0231-R$2DOI
100 1 $aCLAVIJO, F.
245 $aGenetic and phenotypic characterization of Xanthomonas species pathogenic of wheat in Uruguay.$h[electronic resource]
260 $c2022
500 $aArticle history: Accepted for publication/ Posted online on 12 Aug 2021, First Look.
520 $aAbstract: Bacterial diseases affecting wheat production in Uruguay are an issue of growing concern yet remain largely uninvestigated in the region. Surveys of 61 wheat fields carried out from 2017 to 2019 yielded a regional collection of 63 strains identified by 16S rRNA gene analysis as Xanthomonas spp. A real-time PCR protocol using species-specific primers previously reported allowed the identification of 44 strains as X. translucens, the causal agent of bacterial leaf streak (BLS) in wheat and other cereal crops. Multilocus sequence analysis (MLSA) of four housekeeping genes (dnaK, fyuA, gyrB, and rpoD) revealed that these strains were most closely related to X. translucens pv. undulosa, the pathovar that is most commonly associated with BLS of wheat. Multilocus sequence typing (MLST) was applied to examine the genetic diversity among X. translucens strains. Strains were assigned to four different sequence types, three of which have been previously reported globally. Additionally, 17 Xanthomonas strains not belonging to X. translucens were obtained from diseased wheat leaves. Phylogenetic analysis showed that these strains are closely related to Xanthomonas prunicola, and clustered together with previously uncharacterized Xanthomonas strains isolated from wheat in Minnesota, US. In planta pathogenicity assays carried out on a BLS susceptible wheat cultivar showed that X. translucens pv. undulosa strains caused brown necrosis symptoms typical of BLS, while non-translucens Xanthomonas sp. strains elicited an atypical symptom of dry necrosis. These findings suggest that local wheat fields are affected by X. translucens pv. undulosa, and by a new wheat pathogen within the Xanthomonas genus.
650 $aFITOPATOLOGIA
650 $aTRIGO
650 $aTRITICUM AESTIVUM
650 $aURUGUAY
653 $aBacterial leaf streak
653 $aBacterial Pathogens
653 $aMultilocus sequence analysis and typing
653 $aPOPULATION BIOLOGY
653 $aWHEAT
653 $aXanthomonas
700 1 $aCURLAND, R.D.
700 1 $aCROCE, V.
700 1 $aLAPAZ, M.I.
700 1 $aDILL-MACKY, R.
700 1 $aPEREYRA, S.
700 1 $aSIRI, M.I.
773 $tPhytopathology, March 2022, vol. 112 (3): 511-520. Doi: https://doi.org/10.1094/PHYTO-06-21-0231-R
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INIA La Estanzuela (LE) |
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| Registros recuperados : 11 | |
| 7. |  | DILL-MACKY, R.; PEREYRA, S.; BERGSTROM ,GC.; HOFGAARD, I. The role of cultural practices in the control of fusarium head blight of wheat and other cereals. In: International Symposium on Fusarium Head Blight, 5o., International Workshop on Wheat Blast, 2o.,Florianópolis, SC, Brazil,2016. p.80. K10: Session 4-Epidemiology and Management.| Tipo: Abstracts/Resúmenes |
| Biblioteca(s): INIA La Estanzuela. |
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| 9. | | PEREYRA, S.; GARMENDIA, G.; CABRERA, M.; VERO, S.; PIANZZOLA, M.; DILL-MACKY, R. Control biológico de la fusariosis de la espiga de trigo y cebada: resumen. In: TALLER URUGUAYO SOBRE PRODUCCIÓN DE AGENTES MICROBIANOS DE CONTROL BIOLÓGICO, 1., 2006, La Estanzuela, Colonia, UY. Notas del taller y resúmenes de las presentaciones. La Estanzuela: INIA/AgRESEARCH, 2006. p. 12.| Tipo: Abstracts/Resúmenes |
| Biblioteca(s): INIA La Estanzuela. |
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| 10. | | CLAVIJO, F.; CURLAND, R.D.; ROMAN-REYNA, V.; DILL-MACKY, R.; PEREYRA, S.; SIRI, M.I. Caracterización de especies del género Xanthomonas patógenas de trigo en Uruguay. In: Sociedad Uruguaya de Fitopatología Jornada Uruguaya de Fitopatología, 6., Jornada Uruguaya de Protección Vegetal, 4., 21-22 octubre, 2021, Montevideo, Uruguay. Libro de resúmenes. Montevideo (UY): Sociedad Uruguay de Fitopatología (SUFIT), 2021. p. 31. Financiamiento: Proyecto de Investigación Fundamental Fondo Clemente Estable (ANII); Programa Grupos de Investigación I+D de CSIC.| Tipo: Abstracts/Resúmenes |
| Biblioteca(s): INIA Treinta y Tres. |
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| 11. | | CLAVIJO, F.; CURLAND, R.D.; CROCE, V.; LAPAZ, M.I.; DILL-MACKY, R.; PEREYRA, S.; SIRI, M.I. Genetic and phenotypic characterization of Xanthomonas species pathogenic of wheat in Uruguay. Phytopathology, March 2022, vol. 112 (3): 511-520. Doi: https://doi.org/10.1094/PHYTO-06-21-0231-R Article history: Accepted for publication/ Posted online on 12 Aug 2021, First Look.| Tipo: Artículos en Revistas Indexadas Internacionales | Circulación / Nivel : Internacional - -- |
| Biblioteca(s): INIA La Estanzuela. |
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| Registros recuperados : 11 | |
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